Mutant Prevention Concentration Siprofloksasin Terhadap Escherichia coli Patogen dari Kloaka Broiler Secara In Vitro

Mutant prevention concentration (MPC) is an in vitro test used to determine the lowest drug needed inhibit growth of a single-step-mutant bacterial subpopulation. The purpose this study was MPC value ciprofloxacin against pathogenic Escherichia coli obtained range mutant selection windows (MSW) ciprofloxacin. Ciprofloxacin quinolone group that included Highest Priority Critically Important Antimicrobials for Human Medicine but also treatment infections production animals. Twenty-four E. isolates sensitive were tested obtain values and minimum inhibitory (MIC) values. Test MIC get MSW done by method agar dilution. Mueller-Hinton containing standard inoculated with 1010 cfu 104 test. Based on results, 4-64 μg / mL (22.96 ± 19.07 mL) there one isolate which had MPC> 256 mL. These results give wide lower limit 0.25 - 2 µg (0.55 0.37 upper mL). assessment it can be concluded dose animals has allow single-step mutants.